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rabbit anti trpv6 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti trpv6 antibody
    Predicted structures of the mutated <t>TRPV6</t> protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant
    Rabbit Anti Trpv6 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+trpv6+antibody/Anti-TRPV6+Antibody/pmc12924865-155-13-17
    Average 94 stars, based on 47 article reviews
    rabbit anti trpv6 antibody - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice"

    Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

    Journal: Journal of Gastroenterology

    doi: 10.1007/s00535-025-02323-y

    Predicted structures of the mutated TRPV6 protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant
    Figure Legend Snippet: Predicted structures of the mutated TRPV6 protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant

    Techniques Used: Sequencing, Mutagenesis, Variant Assay

    Comparison of different pathogenic genotypes on clinical outcomes. Kaplan–Meier curves showing the cumulative rates of A symptom onset, B pancreatic calcification, C pancreatic exocrine insufficiency, and D diabetes mellitus, according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks
    Figure Legend Snippet: Comparison of different pathogenic genotypes on clinical outcomes. Kaplan–Meier curves showing the cumulative rates of A symptom onset, B pancreatic calcification, C pancreatic exocrine insufficiency, and D diabetes mellitus, according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

    Techniques Used: Comparison

    Timing of the first interventions for pancreatitis and the diagnosis of pancreatic cancer according to pathogenic genotypes. Kaplan–Meier curves showing the cumulative rates of A endoscopic treatment, B surgery, C all interventions, and D pancreatic cancer diagnosis according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks
    Figure Legend Snippet: Timing of the first interventions for pancreatitis and the diagnosis of pancreatic cancer according to pathogenic genotypes. Kaplan–Meier curves showing the cumulative rates of A endoscopic treatment, B surgery, C all interventions, and D pancreatic cancer diagnosis according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

    Techniques Used: Biomarker Discovery

    Caerulein-induced pancreatitis was exacerbated in pancreas-specific Trpv6 conditional knockout mice. A – D Trpv6 floxed mice and pancreas-specific Trpv6 CKO mice received eight hourly intraperitoneal injections of caerulein (100 μg/kg body weight) or saline for two consecutive days. Blood samples were collected at 0 h (just before the first caerulein injection), 8 h, 24 h, and 32 h, and mice were euthanized at either 32 h or 96 h. A Scheme of the experiments. B Blood samples were obtained at the indicated time points, and serum amylase levels were measured. C Representative H&E staining of the pancreas at 32 h. Scale bar = 100 μm. D Representative H&E staining and Sirius Red staining of the pancreas at 96 h after the first caerulein injection. E , F CP was induced by 6-hourly intraperitoneal injections of caerulein (100 μg/kg body weight), 3 days/week for four consecutive weeks. E Scheme of the experiments. F Representative H&E staining and Sirius Red staining of the pancreas after 4 weeks
    Figure Legend Snippet: Caerulein-induced pancreatitis was exacerbated in pancreas-specific Trpv6 conditional knockout mice. A – D Trpv6 floxed mice and pancreas-specific Trpv6 CKO mice received eight hourly intraperitoneal injections of caerulein (100 μg/kg body weight) or saline for two consecutive days. Blood samples were collected at 0 h (just before the first caerulein injection), 8 h, 24 h, and 32 h, and mice were euthanized at either 32 h or 96 h. A Scheme of the experiments. B Blood samples were obtained at the indicated time points, and serum amylase levels were measured. C Representative H&E staining of the pancreas at 32 h. Scale bar = 100 μm. D Representative H&E staining and Sirius Red staining of the pancreas at 96 h after the first caerulein injection. E , F CP was induced by 6-hourly intraperitoneal injections of caerulein (100 μg/kg body weight), 3 days/week for four consecutive weeks. E Scheme of the experiments. F Representative H&E staining and Sirius Red staining of the pancreas after 4 weeks

    Techniques Used: Knock-Out, Saline, Injection, Staining

    Related Articles

    Membrane:

    Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism
    Article Snippet: Immunoblotting SDS-PAGE was performed with the above protein samples (total lysate or plasma membrane) in 6% polyacrylamide gel (Biorad) and transferred into a polyvinylidene fluoride membrane (Immobilon-P, Millipore). .. The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature. .. After being washed three times with PBST, the membrane was treated with anti-mouse IgG-HRP (CST, 1/3,000 dilution) for 30 min at room temperature.

    Incubation:

    Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism
    Article Snippet: Immunoblotting SDS-PAGE was performed with the above protein samples (total lysate or plasma membrane) in 6% polyacrylamide gel (Biorad) and transferred into a polyvinylidene fluoride membrane (Immobilon-P, Millipore). .. The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature. .. After being washed three times with PBST, the membrane was treated with anti-mouse IgG-HRP (CST, 1/3,000 dilution) for 30 min at room temperature.

    Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice
    Article Snippet: .. Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C. .. Immunoreactivity was visualized using a streptavidin–biotin–peroxidase complex detection kit (Histofine Kit; Nichirei Biosciences Inc., Tokyo, Japan) and diaminobenzidine (Dojindo).

    Article Title: Down-regulation of TRPV6 Is Associated With Adverse Prognosis in Hepatocellular Carcinoma Treated With Curative Resection
    Article Snippet: .. The sections were incubated with a rabbit anti-TRPV6 antibody (ACC-036, Alomone labs, Jerusalem, Israel) for 60 min at room temperature, after antigen retrieval with LOW buffer (pH 6.0) in DAKO PT Link (Agilent, Glostrup, Denmark) at 97°C and blocking with Agilent Protein Block, Blocking x0909 (Agilent) for 15 min. Antigen-antibody chromogenic reactions were developed using the Agilent EnVision FLEX Systems K8002 (Agilent) for 30 min. Prostate adenocarcinoma and normal prostate tissues were used as positive and negative controls, respectively, according to the manufacturer’s recommendations. ..

    Article Title: TRPV6-related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice.
    Article Snippet: .. Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C. .. Immunoreactivity was visualized using a streptavidin–biotin–peroxidase complex detection kit (Histofine Kit; Nichirei Biosciences Inc., Tokyo, Japan) and diaminobenzidine (Dojindo).

    Blocking Assay:

    Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism
    Article Snippet: Immunoblotting SDS-PAGE was performed with the above protein samples (total lysate or plasma membrane) in 6% polyacrylamide gel (Biorad) and transferred into a polyvinylidene fluoride membrane (Immobilon-P, Millipore). .. The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature. .. After being washed three times with PBST, the membrane was treated with anti-mouse IgG-HRP (CST, 1/3,000 dilution) for 30 min at room temperature.

    Article Title: Down-regulation of TRPV6 Is Associated With Adverse Prognosis in Hepatocellular Carcinoma Treated With Curative Resection
    Article Snippet: .. The sections were incubated with a rabbit anti-TRPV6 antibody (ACC-036, Alomone labs, Jerusalem, Israel) for 60 min at room temperature, after antigen retrieval with LOW buffer (pH 6.0) in DAKO PT Link (Agilent, Glostrup, Denmark) at 97°C and blocking with Agilent Protein Block, Blocking x0909 (Agilent) for 15 min. Antigen-antibody chromogenic reactions were developed using the Agilent EnVision FLEX Systems K8002 (Agilent) for 30 min. Prostate adenocarcinoma and normal prostate tissues were used as positive and negative controls, respectively, according to the manufacturer’s recommendations. ..

    Article Title: TRPV6 channel modulates proliferation of insulin secreting INS-1E beta cell line.
    Article Snippet: .. Rabbit anti-TRPV6 antibody and TRPV6 blocking peptide was from Alomon Labs (Jerusalem, Israel). .. Mouse anti-β-actin antibody was from Sigma Aldrich.



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    Image Search Results


    Predicted structures of the mutated TRPV6 protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant

    Journal: Journal of Gastroenterology

    Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

    doi: 10.1007/s00535-025-02323-y

    Figure Lengend Snippet: Predicted structures of the mutated TRPV6 protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant

    Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

    Techniques: Sequencing, Mutagenesis, Variant Assay

    Comparison of different pathogenic genotypes on clinical outcomes. Kaplan–Meier curves showing the cumulative rates of A symptom onset, B pancreatic calcification, C pancreatic exocrine insufficiency, and D diabetes mellitus, according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

    Journal: Journal of Gastroenterology

    Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

    doi: 10.1007/s00535-025-02323-y

    Figure Lengend Snippet: Comparison of different pathogenic genotypes on clinical outcomes. Kaplan–Meier curves showing the cumulative rates of A symptom onset, B pancreatic calcification, C pancreatic exocrine insufficiency, and D diabetes mellitus, according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

    Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

    Techniques: Comparison

    Timing of the first interventions for pancreatitis and the diagnosis of pancreatic cancer according to pathogenic genotypes. Kaplan–Meier curves showing the cumulative rates of A endoscopic treatment, B surgery, C all interventions, and D pancreatic cancer diagnosis according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

    Journal: Journal of Gastroenterology

    Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

    doi: 10.1007/s00535-025-02323-y

    Figure Lengend Snippet: Timing of the first interventions for pancreatitis and the diagnosis of pancreatic cancer according to pathogenic genotypes. Kaplan–Meier curves showing the cumulative rates of A endoscopic treatment, B surgery, C all interventions, and D pancreatic cancer diagnosis according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

    Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

    Techniques: Biomarker Discovery

    Caerulein-induced pancreatitis was exacerbated in pancreas-specific Trpv6 conditional knockout mice. A – D Trpv6 floxed mice and pancreas-specific Trpv6 CKO mice received eight hourly intraperitoneal injections of caerulein (100 μg/kg body weight) or saline for two consecutive days. Blood samples were collected at 0 h (just before the first caerulein injection), 8 h, 24 h, and 32 h, and mice were euthanized at either 32 h or 96 h. A Scheme of the experiments. B Blood samples were obtained at the indicated time points, and serum amylase levels were measured. C Representative H&E staining of the pancreas at 32 h. Scale bar = 100 μm. D Representative H&E staining and Sirius Red staining of the pancreas at 96 h after the first caerulein injection. E , F CP was induced by 6-hourly intraperitoneal injections of caerulein (100 μg/kg body weight), 3 days/week for four consecutive weeks. E Scheme of the experiments. F Representative H&E staining and Sirius Red staining of the pancreas after 4 weeks

    Journal: Journal of Gastroenterology

    Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

    doi: 10.1007/s00535-025-02323-y

    Figure Lengend Snippet: Caerulein-induced pancreatitis was exacerbated in pancreas-specific Trpv6 conditional knockout mice. A – D Trpv6 floxed mice and pancreas-specific Trpv6 CKO mice received eight hourly intraperitoneal injections of caerulein (100 μg/kg body weight) or saline for two consecutive days. Blood samples were collected at 0 h (just before the first caerulein injection), 8 h, 24 h, and 32 h, and mice were euthanized at either 32 h or 96 h. A Scheme of the experiments. B Blood samples were obtained at the indicated time points, and serum amylase levels were measured. C Representative H&E staining of the pancreas at 32 h. Scale bar = 100 μm. D Representative H&E staining and Sirius Red staining of the pancreas at 96 h after the first caerulein injection. E , F CP was induced by 6-hourly intraperitoneal injections of caerulein (100 μg/kg body weight), 3 days/week for four consecutive weeks. E Scheme of the experiments. F Representative H&E staining and Sirius Red staining of the pancreas after 4 weeks

    Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

    Techniques: Knock-Out, Saline, Injection, Staining

    Normalized signal density of (A) TRPV6 and (B) PMCA 1 expressions along different parts of the duodenal villus [i.e., villous tip (VT), villus (VL), crypt-villus junction (CVJ), crypt (CR), crypt base (CB)] in rats fed HSD for 3 months. Expression levels of age-matched control groups are normalized to 1. TRPV6, transient receptor potential vanilloid subfamily member 6; PMCA 1 , plasma membrane Ca 2+ -ATPase 1. (n = 5/group; unpaired Student’s t -test; * P < 0.05, *** P < 0.001 vs. age-matched control group).

    Journal: PLOS ONE

    Article Title: Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA 1b in the rat small intestine

    doi: 10.1371/journal.pone.0317415

    Figure Lengend Snippet: Normalized signal density of (A) TRPV6 and (B) PMCA 1 expressions along different parts of the duodenal villus [i.e., villous tip (VT), villus (VL), crypt-villus junction (CVJ), crypt (CR), crypt base (CB)] in rats fed HSD for 3 months. Expression levels of age-matched control groups are normalized to 1. TRPV6, transient receptor potential vanilloid subfamily member 6; PMCA 1 , plasma membrane Ca 2+ -ATPase 1. (n = 5/group; unpaired Student’s t -test; * P < 0.05, *** P < 0.001 vs. age-matched control group).

    Article Snippet: Thereafter, sections were incubated overnight with 1:100 rabbit anti-Ki-67 primary antibody (ab16667; Abcam, Waltham, MA, USA), 1:100 rabbit anti-NKA primary antibody (ab76020; Abcam), 1:100 rabbit anti-TRPV6 primary antibody (SC-28763; Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 rabbit anti-PMCA 1 (ab190355; Abcam), and 1:250 rabbit anti-UGN primary antibody (18113-1-AP; Proteintech, Chicago, IL, USA).

    Techniques: Expressing, Control, Clinical Proteomics, Membrane

    Representative fluorescent photomicrographs of (A) TRPV6 and (B) PMCA 1 distribution along different parts of the duodenal villus in 22-week-old rats as captured by a confocal laser-scanning microscope and a multiphoton microscope, i.e., villous tip (VT), villus (VL), crypt-villus junction (CVJ), crypt (CR), crypt base (CB). Red fluorescent signals represent TRPV6 and PMCA 1 protein expression. Blue fluorescent signals represent nuclei. ** P < 0.01, *** P < 0.001 vs. expression level at VT; # P < 0.05, ### P < 0.001 vs. expression level at VL (n = 5; one-way ANOVA with Tukey’s multiple comparisons test).

    Journal: PLOS ONE

    Article Title: Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA 1b in the rat small intestine

    doi: 10.1371/journal.pone.0317415

    Figure Lengend Snippet: Representative fluorescent photomicrographs of (A) TRPV6 and (B) PMCA 1 distribution along different parts of the duodenal villus in 22-week-old rats as captured by a confocal laser-scanning microscope and a multiphoton microscope, i.e., villous tip (VT), villus (VL), crypt-villus junction (CVJ), crypt (CR), crypt base (CB). Red fluorescent signals represent TRPV6 and PMCA 1 protein expression. Blue fluorescent signals represent nuclei. ** P < 0.01, *** P < 0.001 vs. expression level at VT; # P < 0.05, ### P < 0.001 vs. expression level at VL (n = 5; one-way ANOVA with Tukey’s multiple comparisons test).

    Article Snippet: Thereafter, sections were incubated overnight with 1:100 rabbit anti-Ki-67 primary antibody (ab16667; Abcam, Waltham, MA, USA), 1:100 rabbit anti-NKA primary antibody (ab76020; Abcam), 1:100 rabbit anti-TRPV6 primary antibody (SC-28763; Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 rabbit anti-PMCA 1 (ab190355; Abcam), and 1:250 rabbit anti-UGN primary antibody (18113-1-AP; Proteintech, Chicago, IL, USA).

    Techniques: Laser-Scanning Microscopy, Microscopy, Expressing

    It is likely that an increase in the intestinal absorptive surface area, together with the unregulated TRPV6 and PMCA 1 expression, helped enhance the rate of Ca 2+ absorption as a compensatory mechanism to counterbalance HSD-induced bone loss. Indeed, HSD probably suppressed both bone formation and resorption as indicated by decreased P1NP and CTX1 levels, respectively. Meanwhile, HSD led to greater UGN expression, which might enhance renal Na + excretion. An arrow with a dashed line indicates a hypothetical mechanism. CB, crypt base; CR, crypt; CTX1, C-terminal telopeptide of type 1 collagen; NKA, Na + /K + -ATPase; P1NP, N-terminal propeptide of type 1 procollagen; PMCA 1 , plasma membrane Ca 2+ -ATPase 1; TRPV6, transient receptor potential vanilloid subfamily member 6; UGN, uroguanylin; VT, villous tip.

    Journal: PLOS ONE

    Article Title: Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA 1b in the rat small intestine

    doi: 10.1371/journal.pone.0317415

    Figure Lengend Snippet: It is likely that an increase in the intestinal absorptive surface area, together with the unregulated TRPV6 and PMCA 1 expression, helped enhance the rate of Ca 2+ absorption as a compensatory mechanism to counterbalance HSD-induced bone loss. Indeed, HSD probably suppressed both bone formation and resorption as indicated by decreased P1NP and CTX1 levels, respectively. Meanwhile, HSD led to greater UGN expression, which might enhance renal Na + excretion. An arrow with a dashed line indicates a hypothetical mechanism. CB, crypt base; CR, crypt; CTX1, C-terminal telopeptide of type 1 collagen; NKA, Na + /K + -ATPase; P1NP, N-terminal propeptide of type 1 procollagen; PMCA 1 , plasma membrane Ca 2+ -ATPase 1; TRPV6, transient receptor potential vanilloid subfamily member 6; UGN, uroguanylin; VT, villous tip.

    Article Snippet: Thereafter, sections were incubated overnight with 1:100 rabbit anti-Ki-67 primary antibody (ab16667; Abcam, Waltham, MA, USA), 1:100 rabbit anti-NKA primary antibody (ab76020; Abcam), 1:100 rabbit anti-TRPV6 primary antibody (SC-28763; Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 rabbit anti-PMCA 1 (ab190355; Abcam), and 1:250 rabbit anti-UGN primary antibody (18113-1-AP; Proteintech, Chicago, IL, USA).

    Techniques: Expressing, Clinical Proteomics, Membrane

    Primer sequences for  TRPV6  , PMCA1b , and ACTB (β-actin).

    Journal: Frontiers in Microbiology

    Article Title: Mechanism of Lactiplantibacillus plantarum regulating Ca 2+ affecting the replication of PEDV in small intestinal epithelial cells

    doi: 10.3389/fmicb.2023.1251275

    Figure Lengend Snippet: Primer sequences for TRPV6 , PMCA1b , and ACTB (β-actin).

    Article Snippet: TRPV6 polyclonal rabbit antibodies (Vol: 50 μL, Cat No: 13411-1AP, Dilution: 1:4,000), horseradish peroxidase (HRP)-goat anti-mouse IgG (H + L) (Vol: 100 μL, Cat No: D110087-0100, Dilution: 1:4,000) and β-actin goat anti-rabbit antibody (Vol: 500 μL, Cat No: SA00001-2, Dilution: 1:5,000) were purchased from Proteintech.

    Techniques:

    Validation of the effect of TRPV6 / PMCA1b interference and its impact on intracellular Ca 2+ . (A) PMCA1b protein levels after shPMCA1b expression in IPEC-J2 cells; (B) TRPV6 protein levels after shTRPV6 expression in IPEC-J2 cells; (C) Effect of shTRPV6/shPMCA1b treatment on intracellular Ca 2+ .

    Journal: Frontiers in Microbiology

    Article Title: Mechanism of Lactiplantibacillus plantarum regulating Ca 2+ affecting the replication of PEDV in small intestinal epithelial cells

    doi: 10.3389/fmicb.2023.1251275

    Figure Lengend Snippet: Validation of the effect of TRPV6 / PMCA1b interference and its impact on intracellular Ca 2+ . (A) PMCA1b protein levels after shPMCA1b expression in IPEC-J2 cells; (B) TRPV6 protein levels after shTRPV6 expression in IPEC-J2 cells; (C) Effect of shTRPV6/shPMCA1b treatment on intracellular Ca 2+ .

    Article Snippet: TRPV6 polyclonal rabbit antibodies (Vol: 50 μL, Cat No: 13411-1AP, Dilution: 1:4,000), horseradish peroxidase (HRP)-goat anti-mouse IgG (H + L) (Vol: 100 μL, Cat No: D110087-0100, Dilution: 1:4,000) and β-actin goat anti-rabbit antibody (Vol: 500 μL, Cat No: SA00001-2, Dilution: 1:5,000) were purchased from Proteintech.

    Techniques: Biomarker Discovery, Expressing

    Sequence of  TRPV6  and PMCA1b shRNA interference fragments.

    Journal: Frontiers in Microbiology

    Article Title: Mechanism of Lactiplantibacillus plantarum regulating Ca 2+ affecting the replication of PEDV in small intestinal epithelial cells

    doi: 10.3389/fmicb.2023.1251275

    Figure Lengend Snippet: Sequence of TRPV6 and PMCA1b shRNA interference fragments.

    Article Snippet: TRPV6 polyclonal rabbit antibodies (Vol: 50 μL, Cat No: 13411-1AP, Dilution: 1:4,000), horseradish peroxidase (HRP)-goat anti-mouse IgG (H + L) (Vol: 100 μL, Cat No: D110087-0100, Dilution: 1:4,000) and β-actin goat anti-rabbit antibody (Vol: 500 μL, Cat No: SA00001-2, Dilution: 1:5,000) were purchased from Proteintech.

    Techniques: Sequencing, shRNA

    Effect of interfering with TRPV6 and PMCA1b on PEDV. (A) PEDV-M gene copy number in IPEC-J2 cells after shTRPV6 treatment; (B) PEDV-N protein levels in IPEC-J2 cells after shTRPV6 treatment; (C) PEDV infection of IPEC-J2 after shTRPV6 pretreatment cells; (D) PEDV-M gene copy number in IPEC-J2 cells after shPMCA1b treatment; (E) PEDV protein levels in IPEC-J2 cells after shPMCA1b treatment; (F) change in the viral titer in IPEC-J2 cells infected with PEDV after shPMCA1b pretreatment.

    Journal: Frontiers in Microbiology

    Article Title: Mechanism of Lactiplantibacillus plantarum regulating Ca 2+ affecting the replication of PEDV in small intestinal epithelial cells

    doi: 10.3389/fmicb.2023.1251275

    Figure Lengend Snippet: Effect of interfering with TRPV6 and PMCA1b on PEDV. (A) PEDV-M gene copy number in IPEC-J2 cells after shTRPV6 treatment; (B) PEDV-N protein levels in IPEC-J2 cells after shTRPV6 treatment; (C) PEDV infection of IPEC-J2 after shTRPV6 pretreatment cells; (D) PEDV-M gene copy number in IPEC-J2 cells after shPMCA1b treatment; (E) PEDV protein levels in IPEC-J2 cells after shPMCA1b treatment; (F) change in the viral titer in IPEC-J2 cells infected with PEDV after shPMCA1b pretreatment.

    Article Snippet: TRPV6 polyclonal rabbit antibodies (Vol: 50 μL, Cat No: 13411-1AP, Dilution: 1:4,000), horseradish peroxidase (HRP)-goat anti-mouse IgG (H + L) (Vol: 100 μL, Cat No: D110087-0100, Dilution: 1:4,000) and β-actin goat anti-rabbit antibody (Vol: 500 μL, Cat No: SA00001-2, Dilution: 1:5,000) were purchased from Proteintech.

    Techniques: Infection

    The level of expression of TRPV6 and PMCA1b after LP-1S treatment. (A) TRPV6 mRNA statistics after attack following LP-1S treatment; (B) TRPV6 protein expression levels after attack following by LP-1S treatment; (C) PMCA1b mRNA statistics after attack followingLP-1S treatment; (D) PMCA1b protein levels after attack following LP-1S treatment.

    Journal: Frontiers in Microbiology

    Article Title: Mechanism of Lactiplantibacillus plantarum regulating Ca 2+ affecting the replication of PEDV in small intestinal epithelial cells

    doi: 10.3389/fmicb.2023.1251275

    Figure Lengend Snippet: The level of expression of TRPV6 and PMCA1b after LP-1S treatment. (A) TRPV6 mRNA statistics after attack following LP-1S treatment; (B) TRPV6 protein expression levels after attack following by LP-1S treatment; (C) PMCA1b mRNA statistics after attack followingLP-1S treatment; (D) PMCA1b protein levels after attack following LP-1S treatment.

    Article Snippet: TRPV6 polyclonal rabbit antibodies (Vol: 50 μL, Cat No: 13411-1AP, Dilution: 1:4,000), horseradish peroxidase (HRP)-goat anti-mouse IgG (H + L) (Vol: 100 μL, Cat No: D110087-0100, Dilution: 1:4,000) and β-actin goat anti-rabbit antibody (Vol: 500 μL, Cat No: SA00001-2, Dilution: 1:5,000) were purchased from Proteintech.

    Techniques: Expressing